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F.V. Sances (1), B. Aglave (1), K. Williams (1), C. Boone(1), B. Booker (2), W. Schonborn (3), & E. Rosskopf (3) (1) Florida Ag Research, (2) Ag Metrics Group, (3) US Department of Agriculture (Collaboration Only)
Here, we build on previous work from these and other researchers to develop site-specific “Best Practices” for alternative nematode and pathogenic soil fungi control for Central Florida. Two summer solarization trials took place at the Dover Lab and yields for ASD + Solarization were on par with fumigation. This season we increased precision of applications for manure and molasses and included raised beds or flat ground comparisons.
Flat Ground: In many commercial settings, ASD and Solarization treatments are easier accomplished on flat ground. Here, the chicken manure was applied to the treated area utilizing a cone spreader, followed by mechanical injection of molasses, then the area was disked and plastic mulched.  Methodology
Raised Bed: Planting beds were inoculated with soil-borne diseases, Sting nematodes, and common weed seeds before treatment. Four replicates of five treatments were randomly placed into planting beds previously farmed in strawberries. Following summer solarization/ASD, beds were formed in September (Fumigated) and in October, half of the ASD and Solarization plots with clear plastic were painted black. Flat Ground: Bare ground areas were inoculated with soil-borne diseases, Sting nematodes, and common weed seeds before treatment. Four replicates of four treatments were placed into previously farmed strawberry ground. Each plot comprises one planted bed 120 ft in length. Following summer solarization and ASD plots, beds were formed in September.  Crop Health     Soil Pest Control
Pest control comparison between raised bed and flat ground experiments. Root damage severity due to Macrophomina (a) was greater in the untreated inoculated plots. (b) Fumigated soil had significantly lower incidence of soil pathogens.
Yields       Cumulative Yield data for approximately 6 weeks of harvests at the raised bed (a) and flat ground (b) fields, compared to cumulative annual yield from the previous years’ study at Dover (c) and the Duette, FL, grower site that was a flat ground treatment (d).
F.V. Sances (1), B. Aglave (1), K. Williams (1), C. Boone (1), S. Sances (2), W. Schonborn (3), & E. Rosskopf (3) (1) Florida Ag Research, (2) Ag Metrics Group, (3) US Department of Agriculture (Collaboration Only) Introduction
The recurring strawberry production challenges of managing soil borne pests in commercial Florida strawberry farms have never been greater. In conventionally farmed operations, increasing regulatory pressures on remaining registered chemical soil fumigants, and the costs associated with their use are important factors that reduce the sustainability of the industry going forward. Also, while still in its infancy, organic strawberry production in Florida is now well established, and the 2022-23 season brought numerous examples of severely impacted plantings from both nematodes and soil borne diseases. To meet the demand for domestically produced winter fruit, Florida’s organic strawberry growers have limited options for soil borne pest control and have come to rely on use of new land, field site isolation, high rates of organic soil amendments, and a variety of alternative organically approved soil pest control products with varying levels of efficacy.
 Metagenomic analysis of 16S and ITS sequencing data of soil microbiome. 1149 features detected across 48 samples. Dendrogram determines similarity across samples. Unsupervised clustering (colors) show sample consistency across seasonal timepoints as well as potential outliers (left samples). Processed data was derived from Mr. DNA pipeline. Final timepoint had the clearest separation in soil treatments indicating high reliability of treatment specific species detection. 2022-2023 Data Review
This presentation brings current performance data from these alternative soil management practices, which includes molecular analysis of effects of treatments to the soil food-web, effects on bedding plastics used in solar heating of soil, and a comparison of yield enhancement and pest suppression efficacy of the methods. Cumulative Yield Comparison
Over the 2022-23 season, the period when soil samples were collected, ASD + Solarization resulted in yields on par with the fumigated plots.
Soil Borne Pest Control
Macrophomina and nematode control for solarized plots was improved by “over the top” and ASD methods. Sting nematode suppression in the ASD and “over the top” treatments were on par with fumigant-treated plots. ASD did have a negative effect on certain brands of clear plastic.
 Soil Sample Diversity Data summaries of "stackplot" representations of abundance data from genus level. Each timepoint grouped by treatment and stacked with detectable genera diversity expressed as percent of total abundance. Top 12 out of 349 genera listed. Alternating color bands show diverse genera, thickness is percent abundance ranked from most (bottom) to least (top). EARLY SOIL SAMPLE  MID-SEASON SOIL SAMPLE  LATE-SEASON SOIL SAMPLE  Additional research funding provided by The Florida Strawberry Growers Association Foundation and the USDA
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